▶ Previous Artlcle : #9-2. Factors involved in wound healing
In the last decade, trends in wound treatment have been centered around synthetic dressing materials using polyurethane, which is still commonly used. The recent wound treatment approach focuses on promoting the healing process by supporting the actions of different cells, such as fibroblasts, endothelial cells and macrophages, which play important roles in wound healing. In addition to the cells directly involved in wound healing, changes in the environment of these cells are extensively studied. In particular, the changes in the receptors expressed on the cell wall in response to various types of extracellular matrix, such as collagen, fibrin, fibrinogen, laminin and vitronectin etc. are the focus of interest. In this article, I would like to discuss biological dressing, a more aggressive and promising method of wound treatment.
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History of Keratinocyte Therapy
In 1975, Dr. Howard Green at MIT first succeeded in culturing human keratinocytes. In 1979, he also succeeded in creating a keratinocyte sheet suitable for skin graft by isolating keratinocytes from skin tissue and culturing them for about two weeks. In this experiment, the investigators developed a technique for separating the keratinocyte sheet from the culture vessel by using dispase. Transplantation in humans was attempted in a clinical study in 1981 at the Brigham and Women’s Hospital in Boston, U.S. Since the successful transplantation in a child with burns over 95% of total body surface area (TBSA) at the Shriner’s Hospital in Boston, this technique has been used in clinical practice worldwide.
Figure 1. History of Keratinocyte Therapy
Figure 2. The Mechanism of Cultured Epithelial Autograft
Figure 3. Clinical Application of CEA Sheet
-To be continued-
▶ Next Artlcle : #10-2. Biological Dressing I
