Method

 

 

 

 

The animal study was carried out by Mini Pig, an independent company equipped with sterile breeding facility and operation room. The surgeon and assistants went through a sterilizing shower outside the operation room and put on surgical gowns before entering the operation room. Each person in the room was assigned a specialized task. General anesthesia of animals was carried out using intramuscular injection of Zoletil. After sufficient depth of anesthesia was achieved, the left side of the pig body was left as the control, and the right side was sterilized with rubbing alcohol. All sutures were placed from Zone A to I in a simple interrupted technique.

 

All four pigs received the same procedure on the first day of the study. In this study, the same techniques used in patients in clinical settings were used. The skin on the right flank of the pigs was divided from Zone A to Zone I. The porcine skin used was too thick for the 29G needle and 6-0 PDO thread generally used in humans. The 25G needle and 4-0 PDO thread were used instead (Figure 2).

<Figure 2> Pig skin was divided from Zone A to Zone I.

 

In Zones A to C, we assessed the differences in histological changes and growth factors between deep continuous sutures with short threads and perpendicular insertion of a long thread. In Zone A-1, a 150mm 4-0 PDO thread was laterally placed in subcutaneous fat 1cm apart using a 25G needle. In Zone A-2,a 150mm 4-0 PDO thread was laterally placed 1cm apart using a 25G needle, but was twisted in one direction 10 times. In Zone B-1, a 90mm 4-0 PDO thread was laterally placed 1cm apart in subcutaneous fat using a 25G needle. In Zone B-2, a 90mm 4-0 PDO thread was placed 1cm apart using a 25G needle. The sutures were lateral and placed in the subcutaneous fat. The sutures were twisted in one direction 10 times. In Zone C-1, a 30mm 4-0 PDO thread was perpendicularly placed in subcutaneous fat 1 cm apart using a 25G needle. In Zone C-2, a 30mm 4-0 PDO thread was perpendicularly placed 1cm apart using a 25G needle and the sutures were twisted in one direction 10 times.

 

HELIOSⅡ/LOTUSⅡ/HYPERION – Manufacturer: LASEROPTEK(www.laseroptek.com)

 

From Zone D to G, we assessed the differences in terms of histological changes and growth factors between 1cm apart sutures placed in the dermis and subcutaneous layers according to traditional RE: [N-COG] Skin Rejuvenation techniques, same techniques with 0.5cm intervals and the mesh technique. In Zone D-1, a 90mm 4-0 PDO was placed laterally in subcutaneous fat 1cm apart using a 25G needle. In Zone D-2, a 90mm 4-0 PDO thread was placed laterally in subcutaneous fat 0.5cm apart using 25G needle. In Zone D-3, a 90mm 4-0 PDO thread was placed in subcutaneous fat longitudinally and laterally (mesh), 1 cm apart using a 25G needle(Figure 3, 4). 

<Figure 3> We assessed histological changes and growth factors in Zones D to G.

 

<Figure 4> Simple interrupted sutures were placed laterally and longitudinally in subcutaneous fat to create a mesh pattern.

 

-To be continued